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Thin Layer Chromatography

Melting Point

Simple Distillation

Column Chromatography

Infrared Spectroscopy

Polarimetry

Solubility Testing

Reflux

Extraction

Gas Chromatography-Mass Spectrometry

Classification Tests-Halides

Fractional Distillation

ChemDraw

 

Column Chromatography
(Adapted from Mohrig, pp. 178-187.)

Introduction
Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids when carrying out small-scale experiments. Column chromatography is another solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase). The theory of column chromatography is analogous to that of thin-layer chromatography. The most common adsorbents - silica gel and alumina - are the same ones used in TLC. The sample is dissolved in a small quantity of solvent (the eluent) and applied to the top of the column. The eluent, instead of rising by capillary action up a thin layer, flows down through the column filled with the adsorbent. Just as in TLC, there is an equilibrium established between the solute adsorbed on the silica gel or alumina and the eluting solvent flowing down through the column.

In this experiment, you will be using column chromatography to separate the two components of a binary mixture. You will identify them from their melting points.

Apparatus


Link to detailed procedure for Lab III: Column Chromatography.