What Is It? Why Is It Important? How Do We Measure It? Lab Simulation
Why Is It Important? How Do We Measure It? Lab Simulation
Antibody Staining? How Do We Measure It? Lab Simulation Lab Simulation
 

Antibody Staining

There are many methods that use the exquisite sensitivity of antibodies to locate a cellular component or a specific molecule (a tissue antigen).

Here we focus on the two-step method where the primary antibody is generated against the molecule of interest. The secondary antibody is raised to recognize the primary antibody, and has a fluorescent tag as shown in the drawing on the right.

 

Drawing of the two-step immunocytochemistry method.
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Wellesley College Biology Department :: Last modified: July 11, 2005