Dilution Problems 1. a. How would you make 10mL of a 1:10 dilution of a 1M NaCl solution?      b. What would the final concentration of NaCl be from 1a above?      c. How would you make 80mL of a 1:20 dilution of a 1M NaCl solution?      d. How would you make 50mL of a 1:25 dilutions of a 1M NaCl solution? 2. How would you prepare exactly 6mL of a 1/20 dilution (assume the concentration of your starting solution is "1")? 3. You are provided with an antibody solution (Ab) that has a concentration of 600 microgram (μg) / microlitre (μL). For lab, it is necessary make the following dilutions:      a. 10μL of 600μg/μL Ab + 190μL of buffer to make a 1:20 dilution at _____μg/μL      b. 20μL of 1:20 Ab + 40μL of buffer to make a 1:60 dilution at _____μg/μL      c. 5μL of 1:60 Ab + 5μL of buffer to make a _____dilution at _____μg/μL      d. 10μL of 1:60 Ab + 90μL of buffer to make a_____dilution at _____μg/μL      e. 10μL of 1:60 Ab + 40μL of buffer to make a_____dilution at _____μg/μL      f. 10μL of 1:60 Ab + 10μL of buffer to make a_____dilution at _____μg/μL Click here for the answers

Created by: Cecilia Yu '07
Maintained by: CarolAnn Paul
Date Created: July 1, 2004