Dilution
Problems
1. a. How would
you make 10mL of a 1:10 dilution of a
1M NaCl solution?
b. What would the final concentration of NaCl be
from 1a above?
c. How would you make 80mL of a 1:20 dilution of
a 1M NaCl solution?
d. How would you make 50mL of a 1:25 dilutions
of a 1M NaCl solution?
2. How would you prepare exactly 6mL of a 1/20 dilution (assume the concentration
of your starting solution is "1")?
3. You are provided with an antibody solution (Ab) that has a concentration of
600 microgram (μg) / microlitre (μL). For lab, it is necessary make
the following
dilutions:
a.
10μL of 600μg/μL
Ab + 190μL
of buffer to make a 1:20 dilution at _____μg/μL
b. 20μL of 1:20
Ab + 40μL of buffer to make a 1:60 dilution at _____μg/μL
c. 5μL of 1:60
Ab + 5μL of buffer to make a _____dilution at _____μg/μL
d. 10μL of 1:60
Ab + 90μL of buffer to make a_____dilution at _____μg/μL
e. 10μL of 1:60
Ab + 40μL of buffer to make a_____dilution at _____μg/μL
f. 10μL of 1:60
Ab + 10μL of buffer to make a_____dilution at _____μg/μL
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