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Spec 20 Instructions
The Standard Curve



Volume to Volume

V1C1 = V2C2

Serial Dilutions

Making Solutions

     Percentage by weight
     Percentage by volume
     Molar Solutions
     Buffered solutions


Buffered Solutions

A buffered solution resists changes in pH.

Buffers are used when tissues or organisms are to be maintained at a precise pH, usually between pH 7.2 and 7.4 for human and many animal tissues.

A phosphate buffer is one of the more common buffers used and is made up of a precise mixture of sodium monobasic phosphate (NaH2PO4) and sodium dibasic phosphate (Na2HPO4).


Wellesley College     The Neuron Connection

Created by: Cecilia Yu '07
Maintained by: CarolAnn Paul
Date Created: July 1, 2004
Last Modified: August 25, 2004
Page Expires: August 6, 2006|