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Spec 20 Instructions
The Standard Curve



Volume to Volume

V1C1 = V2C2

Serial Dilutions

Making Solutions

     Percentage by weight
     Percentage by volume
     Molar Solutions
     Buffered solutions


Solutions made using percentage by weight (w/v)

The number of grams in 100mL of solution is indicated by the percentage.
For example, a 1% solution has one gram of solid dissolved in 100mL of solvent. To make this type of solution properly, you should weight 1g and dissolve it in slightly less than 100mL. Once the solids have dissolved, you can bring the volume up to the final 100mL (see animation below).

Making a 1% solution (w/v)

Wellesley College     The Neuron Connection

Created by: Cecilia Yu '07
Maintained by: CarolAnn Paul
Date Created: July 1, 2004
Last Modified: August 25, 2004
Page Expires: August 6, 2006|